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SRX392726: GSM1290066: SS020812 R1.2 co-culture [Shewanella]; Shewanella sp. W3-18-1; RNA-Seq
6 ABI_SOLID (AB 5500xl Genetic Analyzer) runs: 174.5M spots, 8.7G bases, 6.4Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: INTERACTIONS BETWEEN CYANOBACTERIA AND HETEROTROPHS UNDER DIFFERENT TROPHIC CONDITIONS
PRJNA231839 • SRP034523 • All experiments • All runs
show Abstracthide Abstract
Application of genome-scale 'omics approaches to dissect subcellular pathways and regulatory networks governing interspecies interactions is dependent, at least initially, on the availability of model systems with well-annotated genomes and tractable genetics. Overall design: We employed controlled cultivation and next-generation sequencing technology to identify transcriptional responses of euryhaline unicellular cyanobacterium Synechococcus sp. PCC 7002 and a marine facultative aerobe Shewanella putrefaciens W3-18-1 to investigate the effect of C sources and C flux directions on the interactions between these organisms.
Sample: SS020812 R1.2 co-culture [Shewanella]
SAMN02444867 • SRS515716 • All experiments • All runs
Organism: Shewanella sp. W3-18-1
Library:
Instrument: AB 5500xl Genetic Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted using Trizol following by DNaseI treatment using Qiagen RNAseay mini column Template cDNA was prepared using the Applied Biosystems SOLiD® Total RNA-Seq Kit (Life Technologies, Carlsbad, CA) according to manufacturer's protocol. Sequencing was carried using the SOLiDTM 5500XL protocol (Life Technologies).
Experiment attributes:
GEO Accession: GSM1290066
Links:
External link:
Runs: 6 runs, 174.5M spots, 8.7G bases, 6.4Gb
Run# of Spots# of BasesSizePublished
SRR105079429,083,2981.5G1.1Gb2014-01-08
SRR110465629,453,8211.5G1.1Gb2014-01-08
SRR110465728,313,9361.4G1Gb2014-01-08
SRR110465829,043,1921.5G1.1Gb2014-01-08
SRR110465929,575,2321.5G1.1Gb2014-01-08
SRR110466029,019,2161.5G1Gb2014-01-08

ID:
563581

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